Reactive species
Human;Mouse;Rat;Pig(Test by out customer)
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Thymidine kinase cytosolic
Immunogen
The antiserum was produced against synthesized peptide derived from human TK around the phosphorylation site of Ser13. AA range:1-50
Specificity
Phospho-Thymidine Kinase (S13) Polyclonal Antibody detects endogenous levels of Thymidine Kinase protein only when phosphorylated at S13.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/5000.. IF 1:50-200
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
TK1; Thymidine kinase; cytosolic
Background
catalytic activity:ATP + thymidine = ADP + thymidine 5'-phosphate.,miscellaneous:Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.,PTM:Phosphorylated on Ser-13 in mitosis.,similarity:Belongs to the thymidine kinase family.,
Function
catalytic activity:ATP + thymidine = ADP + thymidine 5'-phosphate.,miscellaneous:Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.,PTM:Phosphorylated on Ser-13 in mitosis.,similarity:Belongs to the thymidine kinase family.,
