Antibody type
Monoclonal antibodies
Immunogen
Synthetic Peptide of phospho-MLKL (S358)
Specificity
phospho-MLKL (S358) protein detects endogenous levels of MLKL
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IF: 1:50-200 IHC 1:100-200
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015],
Function
domain:The protein kinase domain is predicted to be catalytically inactive.,similarity:Belongs to the protein kinase superfamily.,similarity:Contains 1 protein kinase domain.,
