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PI3 Kinase P85α Monoclonal Antibody(3B7)

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PI3 Kinase P85α Monoclonal Antibody(3B7)

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Product code: YP-Ab-14242

Promotional price: $ 255

100UL 53UL 10UL

Product introduction

Reactive species

Rat;Mouse

Applications

WB;IHC;IF

Antibody type

Monoclonal antibodies

Gene Name

PIK3R1

Protein name

Phosphatidylinositol 3-kinase regulatory subunit alpha (PI3-kinase regulatory subunit alpha) (PI3K regulatory subunit alpha) (PtdIns-3-kinase regulatory subunit alpha) (Phosphatidylinositol 3-kinase 8

Dalton（DA）

85kD

Immunogen

Recombinant Protein of PI3 Kinase P85α

Specificity

PI3 Kinase P85α protein detects endogenous levels of PI3 Kinase P85α

Constitute

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Monoclonal, Mouse

Dilution rate

WB 1:1000-2000, IHC 1:100-200. IF 1:50-200

Purification process

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

mg/ml

Stockpile

-20°C/1 year

Other name

PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],

Function

disease:Defects in PIK3R1 are a cause of severe insulin resistance.,domain:The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.,function:Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.,PTM:Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.,similarity:Belongs to the PI3K p85 subunit family.,similarity:Contains 1 Rho-GAP domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 2 SH2 domains.,subunit:Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylat

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IP

常规Immunoprecipitation（免疫沉淀 IP）实验操作步骤

WB

蛋白免疫印迹（WB) 实验步骤

PI3 Kinase P85α Monoclonal Antibody(3B7)

Product navigation

PI3 Kinase P85α Monoclonal Antibody(3B7)

Product introduction

Product Details

Customer data and reviews (0)

Citation

Background information

FAQ

Experimental scheme

IP

WB

Primary antibodies

Secondary antibodies

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Recombinant protein

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