Reactive species
Mouse;Rat
Antibody type
Monoclonal antibodies
Protein name
Hydroxyacid oxidase 1
Immunogen
Recombinant Protein of HAO1
Specificity
The antibody detects endogenous HAO1 protein.
Constitute
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Dilution rate
WB: 1:1000-2000 IF 1:200 IHC 1:50-300
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Other name
Hydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GOX
Background
This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008],
Function
catalytic activity:(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2).,cofactor:FMN.,function:Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids.,pathway:Organic acid metabolism; glycolic acid degradation; 3-phospho-D-glyceric acid from glycolic acid: step 1/4.,similarity:Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.,similarity:Contains 1 FMN hydroxy acid dehydrogenase domain.,tissue specificity:Liver.,
