Reactive species
Human;Mouse;Rat;Chicken;Dog;Pig
Antibody type
Monoclonal antibodies
Protein name
Serine/threonine-protein phosphatase PP1-beta catalytic subunit
Immunogen
Purified recombinant human PP1β protein fragments expressed in E.coli.
Specificity
PP1β Monoclonal Antibody detects endogenous levels of PP1β protein.
Constitute
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Dilution rate
Western Blot: 1/1000 - 1/2000. Not yet tested in other applications.
Purification process
Affinity purification
Other name
PPP1CB; Serine/threonine-protein phosphatase PP1-beta catalytic subunit; PP-1B; PPP1CD
Background
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
Function
catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 1 manganese ion per subunit.,domain:The C-terminus is required for CDK2-activation, but not CDK2-binding.,enzyme regulation:The phosphatase activity of the PPP1R15A-PP1 complex toward EIF2S1 is specifically inhibited by Salubrinal, a drug that protects cells from endoplasmic reticulum stress.,function:Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity.,function:Regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. Can activate CDK2 without promoting CDK2 phosphorylation. Mediates cell survival during the DNA damage process throug
