Antibody type
Monoclonal antibodies
Protein name
Tyrosine-protein kinase Tec
Immunogen
Purified recombinant fragment of Tec expressed in E. Coli.
Specificity
Tec Monoclonal Antibody detects endogenous levels of Tec protein.
Constitute
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification process
Affinity purification
Other name
TEC; PSCTK4; Tyrosine-protein kinase Tec
Background
The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome. [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,caution:It is uncertain whether Met-1 is the initiator.,cofactor:Binds 1 zinc ion per subunit.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.,similarity:Contains 1 Btk-type zinc finger.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with INPP5D/SHIP1 and INPPL1/SHIP2.,tissue specificity:Hematopoietic cell lines including myeloid, B-, and T-cell lineages.,
