Reactive species
Human;Mouse;Rat;Monkey
Applications
WB;IHC;IF;FCM;ELISA
Antibody type
Monoclonal antibodies
Protein name
Serine/threonine-protein kinase N2
Immunogen
Purified recombinant fragment of human PRK2 expressed in E. Coli.
Specificity
PRK2 Monoclonal Antibody detects endogenous levels of PRK2 protein.
Constitute
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000.. IF 1:50-200
Purification process
Affinity purification
Other name
PKN2; PRK2; PRKCL2; Serine/threonine-protein kinase N2; PKN gamma; Protein kinase C-like 2; Protein-kinase C-related kinase 2
Background
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C1 domain does not bind the diacylglycerol (DAG).,enzyme regulation:Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids and unsaturated fatty acids. Two specific sites, Thr-816 (activation loop of the kinase domain) and Thr-958 (turn motif), need to be phosphorylated for its full activation.,function:Exhibits a preference for highly basic protein substrates.,PTM:Activated by limited proteolysis with trypsin.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 3 REM (Hr1) repeats.,
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C1 domain does not bind the diacylglycerol (DAG).,enzyme regulation:Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids and unsaturated fatty acids. Two specific sites, Thr-816 (activation loop of the kinase domain) and Thr-958 (turn motif), need to be phosphorylated for its full activation.,function:Exhibits a preference for highly basic protein substrates.,PTM:Activated by limited proteolysis with trypsin.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 3 REM (Hr1) repeats.,
