Reactive species
Human;Rat;Monkey
Applications
WB;IHC;IF;ELISA
Antibody type
Monoclonal antibodies
Protein name
RAC-beta serine/threonine-protein kinase
Immunogen
Purified recombinant fragment of human Akt2 expressed in E. Coli.
Specificity
Akt2 Monoclonal Antibody detects endogenous levels of Akt2 protein.
Constitute
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process
Affinity purification
Other name
AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta
Background
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas.,enzyme regulation:Two specific sites, one in the kinase domain (Thr-309) and the other in the C-terminal regulatory region (Ser-474), need to be phosphorylated for its full activation.,function:General protein kinase capable of phosphorylating several known proteins.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B.,tissue specificity:In all human cell types so far analyzed.,
