Applications
WB;IHC;IF;FCM;ELISA
Antibody type
Monoclonal antibodies
Immunogen
Purified recombinant fragment of human Podocalyxin-like 1 expressed in E. Coli.
Specificity
Podocalyxin-like 1 Monoclonal Antibody detects endogenous levels of Podocalyxin-like 1 protein.
Constitute
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000.. IF 1:50-200
Purification process
Affinity purification
Other name
PODXL; PCLP; PCLP1; Podocalyxin; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1
Background
podocalyxin like(PODXL) Homo sapiens This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008],
Function
function:Functions as an antiadhesin that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion.,PTM:Glycosylated; contains sialic acid.,similarity:Belongs to the podocalyxin family.,tissue specificity:Glomerular epithelium cell (podocyte).,
