Applications
IHC;IF;ELISA
Antibody type
Monoclonal antibodies
Protein name
5'-nucleotidase
Immunogen
Purified recombinant fragment of CD73 expressed in E. Coli.
Specificity
CD73 Monoclonal Antibody detects endogenous levels of CD73 protein.
Constitute
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
IHC: 1/200 - 1/1000. ELISA: 1/10000.. IF 1:50-200
Purification process
Affinity purification
Other name
NT5E; NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; CD antigen CD73
Background
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011],
Function
catalytic activity:A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate.,cofactor:Zinc.,disease:There is a decrease in the activity of NT5 in B-cell chronic lymphocytic leukemia.,function:Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.,similarity:Belongs to the 5'-nucleotidase family.,subunit:Homodimer; disulfide-linked.,
