Applications
WB;IHC;IF;FCM;ELISA
Antibody type
Monoclonal antibodies
Protein name
Transferrin receptor protein 1
Immunogen
Purified recombinant fragment of human CD71 expressed in E. Coli.
Specificity
CD71 Monoclonal Antibody detects endogenous levels of CD71 protein.
Constitute
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification process
Affinity purification
Other name
TFRC; Transferrin receptor protein 1; TR; TfR; TfR1; Trfr; T9; p90; CD antigen CD71
Background
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],
Function
function:Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.,induction:Regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation.,miscellaneous:Canine and feline parvoviruses bind human and feline transferrin receptors and use t
