Antibody type
Monoclonal antibodies
Protein name
T-cell surface glycoprotein CD4
Immunogen
Purified recombinant fragment of human CD4 expressed in E. Coli.
Specificity
CD4 Monoclonal Antibody detects endogenous levels of CD4 protein.
Constitute
Purified antibody in PBS containing 0.03% sodium azide.
Dilution rate
Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification process
Affinity purification
Other name
CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD antigen CD4
Background
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010],
Function
function:Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.,miscellaneous:Primary receptor for HIV-1.,online information:CD4 entry,PTM:Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,similarity:Contains 3 Ig-like C2-type (immunoglobulin-like) domains.,subcellular location:Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.,subunit:Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to t
