Applications
IHC;IF;FCM;ELISA
Antibody type
Monoclonal antibodies
Protein name
Monocyte differentiation antigen CD14
Immunogen
Purified recombinant fragment of human CD14 expressed in E. Coli.
Specificity
CD14 Monoclonal Antibody detects endogenous levels of CD14 protein.
Constitute
Purified antibody in PBS containing 0.03% sodium azide.
Dilution rate
IHC: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000.. IF 1:50-200
Purification process
Affinity purification
Other name
CD14; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD antigen CD14
Background
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010],
Function
function:Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.,online information:CD14 entry,similarity:Contains 11 LRR (leucine-rich) repeats.,subunit:Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.,tissue specificity:Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.,
