Reactive species
Human;Mouse;Rat
Applications
IF;WB;IHC;IP
Antibody type
Monoclonal antibodies
Protein name
Autophagy protein 5 (APG5-like) (Apoptosis-specific protein)
Immunogen
Recombinant Protein of ATG5
Specificity
The antibody detects endogenous ATG5 protein
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IF: 1:50-200 IP: 1:50-200 WB 1:500-2000,IHC-p 1:50-300
Purification process
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
Autophagy protein 5 (APG5-like) (Apoptosis-specific protein)
Background
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015],
Function
function:May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.,function:Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed.,induction:By apoptotic stimuli.,PTM:Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.,similarity:Belongs to the ATG5 family.,subcellular location:Colocalizes with nonmuscle actin.,tissue specificity:Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.,
