Reactive species
Human;Rat;Mouse;Pig
Antibody type
Monoclonal antibodies
Immunogen
Synthetic Peptide of Ubiquitin
Specificity
Ubiquitin protein detects endogenous levels of Ubiquitin
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:1000-2000, IHC 1:100-200 IF 1:200
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
UBB (ubiquitin B) encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBB consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of UBB are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
