Reactive species
Yeast;Human;Mouse;Rat
Antibody type
Monoclonal antibodies
Immunogen
Synthesized peptide derived from Yeast GAPDH
Specificity
This antibody detects endogenous levels of Yeast GAPDH
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.66% sodium azide.
Dilution rate
IHC-p1:50-200 ,WB 1:1000-2000
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
