Reactive species
C. Elegans;Human;Mouse;Rat
Antibody type
Monoclonal antibodies
Gene Name
C. Elegans β-tubulin
Protein name
C. Elegans β-tubulin
Immunogen
Synthesized peptide derived from C. Elegans β-tubulin
Specificity
This antibody detects endogenous levels of C. Elegans β-tubulin
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.10% sodium azide.
Dilution rate
IHC-p1:100-500 ,WB 1:5000-20000
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
