Reactive species
C. Elegans;Human;Mouse;Rat
Antibody type
Monoclonal antibodies
Gene Name
C. Elegans β-actin
Protein name
C. Elegans β-actin
Immunogen
Synthesized peptide derived from C. Elegans β-actin
Specificity
This antibody detects endogenous levels of C. Elegans β-actin
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.07% sodium azide.
Dilution rate
IHC-p1:100-500 ,WB 1:5000-20000
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
