Reactive species
Species independent
Immunogen
Recombinant Protein of Trx-Tag
Specificity
The antibody detects Trx and Trx tag fusion proteins.
Constitute
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
Thioredoxin is a class of small redox proteins known to be present in all organisms. It plays a role in many important biological processes, including redox signaling. The thioredoxin (Trx) fusion E. coli expression system is available to offer soluble expression of normally insoluble or difficult to express proteins. It was reported that a number of mammalian cytokines and growth factors, when expressed as C-terminal trxA fusion proteins, stayed remarkably soluble in the E. coli cytoplasm under certain conditions.