Reactive species
Species independent
Immunogen
Recombinant Protein of RFP-Tag
Specificity
The antibody detects RFP ERFP tag fusion proteins. It isn't reactive against GFP CFP YFP. It is reactive against morange tag weakly.
Constitute
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Dilution rate
WB: 1:10000-20000,IF 1:200-2000
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
RFP (Red Fluorescent Protein), cloned from Discosoma coral (DsRed, DsRed2 or drFP583), is a protein which exhibits bright red fluorescence when exposed to blue light. It is common research practice for biologists to introduce a gene (or a gene chimera) encoding an engineered fluorescent protein into living cells and subsequently visualize the location and dynamics of the gene product using fluorescence microscopy.
