Reactive species
Species independent
Immunogen
Synthetic Peptide of VSV-G-Tag
Specificity
The antibody detects C-terminal, internal, and N-terminal VSV-G fusion proteins.
Constitute
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Dilution rate
WB: 1:5000 IP: 1:200 IF: 1:1000
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. The VSV-G epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods.