Reactive species
Species independent
Immunogen
Synthetic Peptide of V5-Tag
Specificity
The antibody detects C-terminal, internal, and N-terminal V5-tag fusion proteins.
Constitute
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Dilution rate
WB: 1:5000 IP: 1:200 IF: 1:1000
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. This V5-tag antibody is prepared by immunizing synthetic peptide GKPIPNPLLGLDST coupled to KLH.
