Reactive species
Species independent
Applications
WB;ELISA;IP;IF
Immunogen
Synthetic Peptide of HA-Tag
Specificity
The antibody detects C-terminal, internal, and N-terminal HA-tag fusion proteins.
Constitute
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Dilution rate
WB: 1:5000 IP: 1:200 IF: 1:1000;ELISA 1:5000-20000
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.