Reactive species
Species independent
Applications
WB;ELISA;IP;IF
Immunogen
Synthetic Peptide of Myc-Tag
Specificity
The antibody can detect Myc fusion proteins.
Constitute
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Dilution rate
WB: 1:5000 IP: 1:200 IF: 1:1000;ELISA 1:5000-20000
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
Myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.