Reactive species
Human;Mouse;Rat
Antibody type
Monoclonal antibodies
Immunogen
Purified recombinant human WDR77 protein fragments expressed in E.coli.
Specificity
This antibody detects endogenous levels of WDR77 and does not cross-react with related proteins.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
wb dilution 1:1000 icc dilution 1:100
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Other name
2610312E17Rik;Androgen receptor cofactor p44;C79984;HKMT1069;MEP 50;MEP-50;MEP50; MEP50_HUMAN;Methylosome protein 50;MGC2722;Nbla10071;p44;p44/Mep50;RGD1310479;RP11 552M11.3;WD repeat containing protein 77;WD repeat domain 77;WD repeat-containing protein 77;WDR77.
Background
The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015],
Function
developmental stage:Expressed in Leydig cells during fetal testicular development, especially during the second semester. Germ cells expression is detected as early as 10 weeks of gestation.,function:Component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation.,similarity:Contains 5 WD repeats.,subcellular location:Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.,subunit:Component of the methylosome, a 20S complex containing at least PRMT5, CLNS1
