Reactive species
Escherichiacoli
Antibody type
Monoclonal antibodies
Protein name
Thiol:disulfide interchange protein DsbA
Immunogen
Purified recombinant human DsbA protein fragments expressed in E.coli.
Specificity
DsbA Monoclonal Antibody detects endogenous levels of DsbA protein.
Constitute
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Dilution rate
Western Blot: 1/1000 - 1/2000. Not yet tested in other applications.
Purification process
Affinity purification
Background
P0AEG4 periplasmic protein disulfide isomerase I(dsbA) Escherichia coli str. K-12 substr. MG1655 function:Required for disulfide bond formation in some periplasmic proteins such as phoA or ompA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by dsbB. It is required for pilus biogenesis.,similarity:Belongs to the thioredoxin family. DsbA subfamily.,similarity:Contains 1 thioredoxin domain.,
Function
P0AEG4 periplasmic protein disulfide isomerase I(dsbA) Escherichia coli str. K-12 substr. MG1655 function:Required for disulfide bond formation in some periplasmic proteins such as phoA or ompA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by dsbB. It is required for pilus biogenesis.,similarity:Belongs to the thioredoxin family. DsbA subfamily.,similarity:Contains 1 thioredoxin domain.,
