Reactive species
Species independent
Immunogen
6X His synthetic peptide conjugated to KLH.
Specificity
His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Background
The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.
