Reactive species
Human;Rat;Mouse;
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Nuclear autoantigen Sp-100
Immunogen
Synthesized peptide derived from SP-100 . at AA range: 250-330
Specificity
SP-100 Polyclonal Antibody detects endogenous levels of SP-100 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
SP100; Nuclear autoantigen Sp-100; Lysp100b; Nuclear dot-associated Sp100 protein; Speckled 100 kDa
Background
This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011],
Function
disease:This antigen is recognized by autoantibodies from patients with primary biliary cirrhosis (PBC).,domain:Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.,domain:The HSR domain is important for the nuclear body targeting as well as for the dimerization.,function:May play a role in the control of gene expression.,induction:By interferon.,miscellaneous:The major isoform Sp100-A, has a calculated MW of 54 kDa, but exhibits aberrant electrophoretic mobilities, with an apparent MW OF 100 kDa.,PTM:Phosphorylated.,PTM:Sumoylated. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting.,similarity:Contains 1 HSR domain.,similarity:Contains 1 SA
