Reactive species
Human;Mouse
Antibody type
Polyclonal Antibody
Protein name
RUN and FYVE domain-containing protein 1
Immunogen
The antiserum was produced against synthesized peptide derived from human RUFY1. AA range:139-188
Specificity
RUFY1 Polyclonal Antibody detects endogenous levels of RUFY1 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
RUFY1; RABIP4; ZFYVE12; RUN and FYVE domain-containing protein 1; FYVE-finger protein EIP1; La-binding protein 1; Rab4-interacting protein; Zinc finger FYVE domain-containing protein 12
Background
RUN and FYVE domain containing 1(RUFY1) Homo sapiens This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011],
Function
domain:The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate.,function:Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.,similarity:Contains 1 FYVE-type zinc finger.,similarity:Contains 1 RUN domain.,subunit:Interacts with BMX. May interact with SSB. Interacts with RAB4 and RAB5 that have been activated by GTP-binding.,tissue specificity:Broadly expressed, with highest levels in lung, testis, kidney and brain.,
