Reactive species
Human;Rat;Mouse;
Antibody type
Polyclonal Antibody
Protein name
Alanine aminotransferase 2
Immunogen
Synthesized peptide derived from GPT2 . at AA range: 190-270
Specificity
GPT2 Polyclonal Antibody detects endogenous levels of GPT2 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
GPT2; AAT2; ALT2; Alanine aminotransferase 2; ALT2; Glutamate pyruvate transaminase 2; GPT 2; Glutamic--alanine transaminase 2; Glutamic--pyruvic transaminase 2
Background
This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015],
Function
catalytic activity:L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.,cofactor:Pyridoxal phosphate.,pathway:Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.,similarity:Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.,subunit:Homodimer.,tissue specificity:Expressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast.,
