Reactive species
Human;Mouse
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Docking protein 4
Immunogen
The antiserum was produced against synthesized peptide derived from human DOK4. AA range:11-60
Specificity
Dok-4 Polyclonal Antibody detects endogenous levels of Dok-4 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
DOK4; Docking protein 4; Downstream of tyrosine kinase 4; Insulin receptor substrate 5; IRS-5; IRS5
Background
domain:PTB domain mediates receptor interaction.,function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.,PTM:Phosphorylated on tyrosine residues in response to insulin, IGF1 or RET stimulation.,similarity:Belongs to the DOK family. Type B subfamily.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:Interacts with RET and TEK/TIE2. Interaction with RET is mediated through the PTB domain and requires phosphorylation of RET 'Tyr-1062'.,tissue specificity:Widely expressed. High expression in skeletal muscle, heart, kidney and liver. Weaker expression in spleen, lung and small intestine, brain, heart and. Expressed in both resting and activated peripheral blood T-cells.,
Function
domain:PTB domain mediates receptor interaction.,function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.,PTM:Phosphorylated on tyrosine residues in response to insulin, IGF1 or RET stimulation.,similarity:Belongs to the DOK family. Type B subfamily.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:Interacts with RET and TEK/TIE2. Interaction with RET is mediated through the PTB domain and requires phosphorylation of RET 'Tyr-1062'.,tissue specificity:Widely express
