Reactive species
Human;Rat;Mouse;
Applications
IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Centrosome-associated protein 350
Immunogen
The antiserum was produced against synthesized peptide derived from human CEP35. AA range:671-720
Specificity
CEP350 Polyclonal Antibody detects endogenous levels of CEP350 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
CEP350; CAP350; KIAA0480; GM133; Centrosome-associated protein 350; Cep350; Centrosome-associated protein of 350 kDa
Background
centrosomal protein 350(CEP350) Homo sapiens The product of this gene is a large protein with a CAP-Gly domain typically found in cytoskeleton-associated proteins. The encoded protein primarily localizes to the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. The encoded protein directly interacts with another large centrosomal protein and is required to anchor microtubules at the centrosome. It is also implicated in the regulation of a class of nuclear hormone receptors in the nucleus. Several alternatively spliced transcript variants have been found, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008],
Function
function:Required for anchoring microtubules to the centrosomes. Required for the integrity of the microtubule network. Recruits PPARA to discrete subcellular compartments and thereby modulates PPARA activity.,PTM:Phosphorylated during mitosis.,similarity:Contains 1 CAP-Gly domain.,subcellular location:Associated with mitotic spindles. Nuclear, in discrete foci. Associated with intermediate filaments.,subunit:Part of a ternary complex that contains CEP350, FGFR1OP and MAPRE1. Interacts directly with FGFR1OP via its C-terminus. Interacts with NR1H3, PPARA, PPARD and PPARG.,tissue specificity:Detected in heart, brain, skeletal muscle, testis, placenta, lung, liver, kidney and pancreas.,
