Reactive species
Human;Rat;Mouse;
Antibody type
Polyclonal Antibody
Protein name
ADP-ribosylation factor GTPase-activating protein 3
Immunogen
Synthesized peptide derived from ARF GAP3 . at AA range: 280-360
Specificity
ARF GAP3 Polyclonal Antibody detects endogenous levels of ARF GAP3 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
ARFGAP3; ARFGAP1; ADP-ribosylation factor GTPase-activating protein 3; ARF GAP 3
Background
The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008],
Function
caution:Was originally (PubMed:10704287) termed ARFGAP1.,developmental stage:Expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development.,enzyme regulation:GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2).,function:GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.,similarity:Contains 1 Arf-GAP domain.,subcellular location:Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.,tissue specificity:Widely expressed. Highest expression in endocrine glands (pancreas, pituary gland, salivary gland, and prostate) and testis with a much higher expression
