Reactive species
Human;Mouse;Rat
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
ADP-dependent glucokinase
Immunogen
The antiserum was produced against synthesized peptide derived from human ADPGK. AA range:241-290
Specificity
ADP-GK Polyclonal Antibody detects endogenous levels of ADP-GK protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
ADPGK; PSEC0260; ADP-dependent glucokinase; ADP-GK; ADPGK; RbBP-35
Background
ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).[supplied by OMIM, Mar 2008],
Function
catalytic activity:ADP + D-glucose = AMP + D-glucose 6-phosphate.,cofactor:Binds 1 magnesium ion per subunit.,function:Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency.,pathway:Carbohydrate degradation; glycolysis.,similarity:Belongs to the ADP-dependent glucokinase family.,similarity:Contains 1 ADPK (ADP-dependent kinase) domain.,subunit:Monomer.,
