Reactive species
Human;Mouse;Rat
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
GRB2-associated-binding protein 1
Immunogen
The antiserum was produced against synthesized peptide derived from human GAB1 around the phosphorylation site of Tyr659. AA range:644-674
Specificity
Phospho-Gab 1 (Y659) Polyclonal Antibody detects endogenous levels of Gab 1 protein only when phosphorylated at Y659.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
GAB1; GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1
Background
GRB2 associated binding protein 1(GAB1) Homo sapiens The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],
Function
function:Probably involved in EGF and insulin receptor signaling.,PTM:Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.,similarity:Belongs to the GAB family.,similarity:Contains 1 PH domain.,subunit:Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2.,
