Reactive species
Human;Mouse;Rat
Antibody type
Monoclonal antibodies
Immunogen
Purified recombinant human SirT2 protein expressed in E.coli.
Specificity
This antibody detects endogenous levels of SirT2 and does not cross-react with related proteins.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
wb 1:1000. IF 1:50-200
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Other name
FLJ35621;FLJ37491;NAD dependent deacetylase sirtuin 2;NAD-dependent deacetylase sirtuin-2;NAD-dependent protein deacetylase sirtuin-2;Regulatory protein SIR2 homolog 2;Silencing information regulator 2 like;Silent information regulator 2;Silent mating type information regulation 2;Silent mating type information regulation 2 homolog;SIR 2;SIR2;SIR2 like;SIR2 like protein 2;Sir2 related protein type 2;SIR2, S. cerevisiae, homolog-loke 2;SIR2-like protein 2;SIR2L;SIR2L2;SIRT 2;SIRT2;SIRT2_HUMAN;Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae);Sirtuin 2;Sirtuin type 2;Sirtuin2.
Background
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene. [provided by RefSeq, Jul 2010],
Function
catalytic activity:NAD(+) + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.,cofactor:Binds 1 zinc ion per subunit.,developmental stage:Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.,enzyme regulation:Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide.,function:NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo.,PTM:Phosphorylated at the G2/M transition of the cell cycle.,similarity:Belongs to the sirtuin family.,similarity:Contains 1 deacetylase sirtuin-type domain.,subcellular location:Colocalizes with microtubules.,subunit:Interacts with HDAC6, suggesting that these proteins belong to a la
