Applications
WB;IHC;IF;ELISA
Antibody type
Monoclonal antibodies
Protein name
Nuclear receptor corepressor 1
Immunogen
Purified recombinant fragment of N-CoR (aa1-192) expressed in E. Coli.
Specificity
N-CoR Monoclonal Antibody detects endogenous levels of N-CoR protein.
Constitute
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/200 - 1/1000. ELISA: 1/10000.. IF 1:50-200
Purification process
Affinity purification
Other name
NCOR1; KIAA1047; Nuclear receptor corepressor 1; N-CoR; N-CoR1
Background
This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.[provided by RefSeq, Jun 2010],
Function
domain:The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity.,domain:The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).,function:Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.,PTM:Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.,similarity:Belongs to the N-CoR nuclear receptor corepres
