Reactive species
Human;Mouse;Rat
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Immunogen
The antiserum was produced against synthesized peptide derived from human Calmodulin around the phosphorylation site of Thr79 and Ser81. AA range:46-95
Specificity
Phospho-Calmodulin (T80/S82) Polyclonal Antibody detects endogenous levels of Calmodulin protein only when phosphorylated at T80/S82.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
CALM1; CALM; CAM; CAM1; CALM2; CAM2; CAMB; CALM3; CALML2; CAM3; CAMC; CAMIII; Calmodulin; CaM
Background
This gene encodes a member of the EF-hand calcium-binding protein family. It is one of three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009],
Function
function:Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.,miscellaneous:This protein has four functional calcium-binding sites.,PTM:Phosphorylation results in a decreased activity.,PTM:Ubiquitination results in a strongly decreased activity.,similarity:Belongs to the calmodulin family.,similarity:Contains 4 EF-hand domains.,subcellular location:Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.,subunit:Interacts with MYO1C (By similarity). Interacts with CEP97, CEP110, TTN/titin and SRY.,
