Reactive species
Human;Mouse;Rat
Antibody type
Polyclonal Antibody
Immunogen
The antiserum was produced against synthesized peptide derived from human Ezrin around the phosphorylation site of Thr566. AA range:534-583
Specificity
Phospho-Ezrin (T566) Polyclonal Antibody detects endogenous levels of Ezrin protein only when phosphorylated at T566.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81
Background
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008],
Function
developmental stage:Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.,function:Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.,PTM:Phosphorylated by tyrosine-protein kinases.,similarity:Contains 1 FERM domain.,subcellular location:Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein (cytoplasmic side).,subunit:Interacts with MPP5 (By similarity). Interacts with SLC9A3R1 and SCYL3/PACE1. Interacts with PLEKHG6. Interacts with NGX6.,tissue s
