Reactive species
Human;Mouse;Rat
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Gene Name
AMY1A/AMY1B/AMY1C/AMY2A/AMY2B
Protein name
Alpha-amylase 1/Pancreatic alpha-amylase/Alpha-amylase 2B
Immunogen
The antiserum was produced against synthesized peptide derived from the N-terminal region of human AMY1/2. AA range:61-110
Specificity
Amylase Polyclonal Antibody detects endogenous levels of Amylase protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000.. IF 1:50-200
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
AMY1A; AMY1; AMY1B; AMY1; AMY1C; AMY1; Alpha-amylase 1; 1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase; AMY2A; Pancreatic alpha-amylase; PA;1,4-alpha-D-glucan glucanohydrolase; AMY2B; Alpha-amylase 2B; 1,4-alpha-D-glucan glucanohydrolase 2B; Carcinoid alpha-amylase
Background
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],
Function
catalytic activity:Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.,cofactor:Binds 1 calcium ion per subunit.,cofactor:Binds 1 chloride ion per subunit.,online information:Amylase entry,similarity:Belongs to the glycosyl hydrolase 13 family.,subunit:Monomer.,
