Reactive species
Human;Rat;Mouse;
Applications
IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Aldo-keto reductase family 1 member C1
Immunogen
Synthesized peptide derived from DD1 . at AA range: 30-110
Specificity
DD1 Polyclonal Antibody detects endogenous levels of DD1 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
AKR1C1; DDH; DDH1; Aldo-keto reductase family 1 member C1; 20-alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; Chlordecone reductase homolog HAKRC; Dihydrodiol dehydrogenase 1/2; DD1/DD2; High-affinity hepatic bile acid-binding protein; HB
Background
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008],
Function
catalytic activity:17-alpha,20-alpha-dihydroxypregn-4-en-3-one + NAD(P)(+) = 17-alpha-hydroxyprogesterone + NAD(P)H.,catalytic activity:Indan-1-ol + NAD(P)(+) = indanone + NAD(P)H.,catalytic activity:Trans-1,2-dihydrobenzene-1,2-diol + NADP(+) = catechol + NADPH.,enzyme regulation:Inhibited by hexestrol with an IC(50) of 9.5 uM, 1,10-phenanthroline with an IC(50) of 55 uM, 1,7-phenanthroline with an IC(50) of 72 uM, flufenamic acid with an IC(50) of 6.0 uM, indomethacin with an IC(50) of 140 uM, ibuprofen with an IC(50) of 950 uM, lithocholic acid with an IC(50) of 25 uM, ursodeoxycholic acid with an IC(50) of 340 uM and chenodeoxycholic acid with an IC(50) of 570 uM.,function:Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile a
