Reactive species
Human;Mouse;Rat
Antibody type
Polyclonal Antibody
Protein name
Nucleolar and coiled-body phosphoprotein 1
Dalton（DA）
74,130KD(Nucleolar phosphoprotein p130)
Immunogen
Synthesized peptide derived from Nopp140 . at AA range: 620-700
Specificity
Nopp140 Polyclonal Antibody detects endogenous levels of Nopp140 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
NOLC1; KIAA0035; NS5ATP13; Nucleolar and coiled-body phosphoprotein 1; 140 kDa nucleolar phosphoprotein; Nopp140; Hepatitis C virus NS5A-transactivated protein 13; HCV NS5A-transactivated protein 13; Nucleolar 130 kDa protein; Nucleolar pho
Background
function:Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.,PTM:Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. There is evidence suggesting that CDC2 kinase phosphorylates p130 at the M-phase.,similarity:Contains 1 LisH domain.,subcellular location:Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase.,subunit:Interacts with RNA polymerase I 194 kDa subunit (RPA194) and with casein kinase-II.,
Function
function:Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.,PTM:Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. There is evidence suggesting that CDC2 kinase phosphorylates p130 at the M-phase.,similarity:Contains 1 LisH domain.,subcellular location:Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase.,subunit:Interacts with RNA polymerase I 194 kDa subunit (RPA194) and with casein kinase-II.,
