Reactive species
Human;Rat;Mouse;
Antibody type
Polyclonal Antibody
Protein name
Condensin complex subunit 3
Immunogen
The antiserum was produced against synthesized peptide derived from human NCAPG. AA range:951-1000
Specificity
hCAP-G Polyclonal Antibody detects endogenous levels of hCAP-G protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
NCAPG; CAPG; NYMEL3; Condensin complex subunit 3; Chromosome-associated protein G; Condensin subunit CAP-G; hCAP-G; Melanoma antigen NY-MEL-3; Non-SMC condensin I complex subunit G; XCAP-G homolog
Background
This gene encodes a subunit of the condensin complex, which is responsible for the condensation and stabilization of chromosomes during mitosis and meiosis. Phosphorylation of the encoded protein activates the condensin complex. There are pseudogenes for this gene on chromosomes 8 and 15. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012],
Function
function:Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.,miscellaneous:Overexpressed in some cancer lines and some tumor cells.,PTM:Phosphorylated by CDC2. Its phosphorylation, as well as that of NCAPD2 and NCAPH subunits, activates the condensin complex and is required for chromosome condensation.,sequence caution:Wrong choice of frame.,similarity:Belongs to the CND3 (condensin subunit 3) family.,similarity:Contains 10 HEAT repeats.,subcellular location:In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated
