Reactive species
Human;Mouse;Rat
Applications
WB;IHC;IF;CoIP;ELISA
Antibody type
Polyclonal Antibody
Protein name
Nuclear receptor coactivator 4
Immunogen
Synthesized peptide derived from ARA70 . at AA range: 320-400
Specificity
ARA70 Polyclonal Antibody detects endogenous levels of ARA70 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/40000. IP 1:50-200Not yet tested in other applications.. IF 1:50-200
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
NCOA4; ARA70; ELE1; RFG; Nuclear receptor coactivator 4; NCoA-4; Androgen receptor coactivator 70 kDa protein; 70 kDa AR-activator; 70 kDa androgen receptor coactivator; Androgen receptor-associated protein of 70 kDa; Ret-activating protein
Background
This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009],
Function
alternative products:Additional isoforms seem to exist,disease:A chromosomal aberration involving NCOA4 is a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas.,function:Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma.,polymorphism:Polymorphisms are specific to isoform alpha. Not found in isoform beta. Could be encoded by two different genes.,subunit:Interacts with the androgen receptor and the retinoid X receptor (RXR) in a ligand-dependent manner.,tissue specificity:Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform beta is only expressed in testis
