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Rpb1 CTD (phospho-Thr4) rabbit pAb

Product code: YP-Ab-01466
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Product introduction

Reactive species
Human;Mouse;Rat
Applications
WB
Antibody type
Polyclonal Antibody
Gene Name
POLR2A POLR2
Protein name
Rpb1 CTD (Thr4)
Dalton(DA)
250kD
Immunogen
Synthesized phosho peptide around human Rpb1 CTD (Thr4)
Specificity
This antibody detects endogenous levels of Human Mouse Rat Rpb1 CTD (phospho-Thr4)
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB 1:1000-2000
Purification process
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration
1 mg/ml
Stockpile
-20°C/1 year
Other name
DNA-directed RNA polymerase II subunit RPB1 (RNA polymerase II subunit B1) (EC 2.7.7.6) (DNA-directed RNA polymerase II subunit A) (DNA-directed RNA polymerase III largest subunit) (RNA-directed RNA polymerase II subunit RPB1) (EC 2.7.7.48)
Background
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008],
Function
catalytic activity:Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).,function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of

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