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Elk-1 (phospho Thr417) Polyclonal Antibody

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Elk-1 (phospho Thr417) Polyclonal Antibody

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Product code: YP-Ab-01239

Promotional price: $ 255

100UL 53UL 10UL

Product introduction

Reactive species

Human;Mouse;Rat

Applications

IHC;IF;IP;ELISA

Antibody type

Polyclonal Antibody

Gene Name

ELK1

Protein name

ETS domain-containing protein Elk-1

Dalton（DA）

Immunogen

The antiserum was produced against synthesized peptide derived from human Elk1 around the phosphorylation site of Thr417. AA range:379-428

Specificity

Phospho-Elk-1 (T417) Polyclonal Antibody detects endogenous levels of Elk-1 protein only when phosphorylated at T417.

Constitute

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Polyclonal, Rabbit,IgG

Dilution rate

IHC: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000.. IF 1:50-200

Purification process

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Stockpile

-20°C/1 year

Other name

ELK1; ETS domain-containing protein Elk-1

Background

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012],

Function

function:Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.,PTM:On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.,PTM:Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Intera

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IP

常规Immunoprecipitation（免疫沉淀 IP）实验操作步骤

WB

蛋白免疫印迹（WB) 实验步骤

Elk-1 (phospho Thr417) Polyclonal Antibody

Product navigation

Elk-1 (phospho Thr417) Polyclonal Antibody

Product introduction

Product Details

Customer data and reviews (0)

Citation

Background information

FAQ

Experimental scheme

IP

WB

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