Reactive species
Human;Rat;Mouse;Monkey
Antibody type
Monoclonal antibodies
Immunogen
Purified recombinant human DDX3 protein fragments expressed in E.coli.
Specificity
This antibody detects endogenous levels of DDX3 and does not cross-react with related proteins.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
wb dilution 1:1000 icc dilution 1:200
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Other name
ATP dependent RNA helicase DDX3X;ATP-dependent RNA helicase DDX3X;CAP Rf;DBX;DDX14;DDX3X;DDX3X_HUMAN;DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked;DEAD box;DEAD box protein 3;DEAD box protein 3 X-chromosomal;DEAD box X isoform;DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3;DEAD/H box 3;DEAD/H box 3, X-linked;Fibroblast Growth Factor Inducible 14;Fin14;Helicase like protein 2;Helicase-like protein 2;HLP2;X isoform;X-chromosomal.
Background
The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarit
Function
function:ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.,similarity:Belongs to the DEAD box helicase family.,similarity:Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,subcellular location:Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.,subunit:Found in a complex with Rev and XPO1. Interacts with XPO1 and TDRD3. Interacts with HCV core protein.,
