Antibody type
Monoclonal antibodies
Immunogen
Purified recombinant human FOXO1(C-terminus) protein fragments expressed in E.coli.
Specificity
This antibody detects endogenous levels of FOXO1(C-terminus) and does not cross-react with related proteins.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Other name
FKH 1;FKH1;FKH1;FKHR;FKHR;Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma);Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma);Forkhead box O1;Forkhead box protein O1; Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;Forkhead, Drosophila, homolog of, in rhabdomyosarcoma;FOXO1;FOXO1;FOXO1_HUMAN;FOXO1A;OTTHUMP00000018301.
Background
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008],
Function
disease:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.,function:Transcription factor.,PTM:Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 fork-head DNA-binding domain.,subcellular location:Shuttles betw
