Reactive species
Human;Mouse;Rat;Bovine;Chicken;Dog;Pig
Antibody type
Monoclonal antibodies
Protein name
Transcription initiation factor IIB
Immunogen
Purified recombinant human TFIIB protein fragments expressed in E.coli.
Specificity
TFIIB Monoclonal Antibody detects endogenous levels of TFIIB protein.
Constitute
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Dilution rate
Western Blot: 1/1000 - 1/2000. Immunohistochemistry: 1/500 - 1/1000. Immunofluorescence: 1/100 - 1/500. Not yet tested in other applications.
Purification process
Affinity purification
Other name
GTF2B; TF2B; TFIIB; Transcription initiation factor IIB; General transcription factor TFIIB; S300-II
Background
general transcription factor IIB(GTF2B) Homo sapiens This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008],
Function
cofactor:Binds 1 zinc ion per subunit.,function:General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.,similarity:Belongs to the TFIIB family.,similarity:Contains 1 TFIIB-type zinc finger.,subunit:Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV-1 Vpr. Interacts with GPBP1 (By similarity). Interacts with Epstein-Barr virus EBNA2.,
