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Histone H3 (Acetyl Lys23) Polyclonal Antibody

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Histone H3 (Acetyl Lys23) Polyclonal Antibody

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Product code: YP-Ab-00837

Promotional price: $ 255

100UL 53UL 10UL

Product introduction

Reactive species

Human;Mouse;Rat

Applications

WB;IF;ELISA

Antibody type

Polyclonal Antibody

Gene Name

HIST1H3A/HIST1H3B/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B

Protein name

Histone H3.1/Histone H3.2/Histone H3.3

Dalton（DA）

17kD

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys23. AA range:-9-40

Specificity

Acetyl-Histone H3 (K23) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when acetylated at K23.

Constitute

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Polyclonal, Rabbit,IgG

Dilution rate

Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Purification process

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Stockpile

-20°C/1 year

Other name

HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3;H3k23AC

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Function

caution:Was originally (PubMed:2587222) thought to originate from mouse.,developmental stage:Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.,function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589,miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).,PTM:Acetylation is generally l

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IP

常规Immunoprecipitation（免疫沉淀 IP）实验操作步骤

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蛋白免疫印迹（WB) 实验步骤

Histone H3 (Acetyl Lys23) Polyclonal Antibody

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Histone H3 (Acetyl Lys23) Polyclonal Antibody

Product introduction

Product Details

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Background information

FAQ

Experimental scheme

IP

WB

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